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Intrinsic Hepcidin IDx™ EDTA ELISA Kit

$838.00

Species: Human Analyte: Hepcidin
Range: 0.55 – 400 ng/ml Sensitivity: 0.275 ng/ml
SKU: ICE-004 Category: Tags: , , , ,
Catalog NumberICE-004
Quantity96 Wells
ApplicationELISA, EIA, RUO
MethodCompetitive
Antibody TypeMonoclonal
Target SpeciesHuman
AnalyteHepcidin-25, HAMP, HEPC, LEAP1
Assay time1hr 45m
Detection methodColorimetric
Sample type{"field_5fb6846bc1588":"Serum"}
Sample volume required per well24
Detection range0.55 – 400 ng/ml
Sensitivity0.275ng/ml
Regulatory statusResearch Use Only ELISA
Storage requirements4C

Typical Data

Hepcidin StandardsConcentration (ng/mL)Absorbance (450nm)
102.36
2513.5
31.511
41.87
51.34
61.87
71.3342
81.1214

Precision

Intra assay PrecisionInter assay Precision
Sample123123
nTBDTBDTBDTBDTBDTBD
Mean (ng/ml)TBDTBDTBDTBDTBDTBD
Standard DeviationTBDTBDTBDTBDTBDTBD
CV(%)TBDTBDTBDTBDTBDTBD

Linearity

Serum
1:2Range (%)TBD
Average (%)98
1:4Range (%)TBD
Average (%)98

Recovery

Sample TypeRange(%)Average Recovery(%)
SerumTBDTBD

Description

The Intrinsic Hepcidin IDx™ EDTA ELISA is designed for quantification of hepcidin-25 in human EDTA plasma samples only. This kit does not contain mercaptoethanol, azide- or mercury-based preservatives.

Hepcidin was first discovered as an antimicrobial peptide produced in the liver and then identified as the peptide hormone that regulates extracellular iron in response to changes in dietary and systemic iron load, anemia, hypoxia, and erythropoiesis. Hepcidin is an acute phase protein that is elevated in inflammation with IL-6 acting as the principal regulator of hepcidin during inflammation. Hepcidin inhibits cellular iron efflux by binding to and inducing degradation of the sole known iron channel, ferroportin.

U.S. Pat. No. 9,657,098; NZ Pat. No. 631098; Pat. Pending in the US and abroad.

ICE-004 Product Insert v3

Questions? Please email us: custserv@intrinsiclifesciences.com

 

Additional information

Weight 10 oz
Dimensions 5 × 7 × 3.5 in