PRODUCTS
Intrinsic Hepcidin IDx™ EDTA ELISA Kit
$838.00
| Species: Human | Analyte: Hepcidin |
| Range: 0.55 – 400 ng/ml | Sensitivity: 0.275 ng/ml |
| Catalog Number | ICE-004 |
| Quantity | 96 Wells |
| Application | ELISA, EIA, RUO |
| Method | Competitive |
| Antibody Type | Monoclonal |
| Target Species | Human |
| Analyte | Hepcidin-25, HAMP, HEPC, LEAP1 |
| Assay time | 1hr 45m |
| Detection method | Colorimetric |
| Sample type | {"field_5fb6846bc1588":"Serum"} |
| Sample volume required per well | 24 |
| Detection range | 0.55 – 400 ng/ml |
| Sensitivity | 0.275ng/ml |
| Regulatory status | Research Use Only ELISA |
| Storage requirements | 4C |
Typical Data
| Hepcidin Standards | Concentration (ng/mL) | Absorbance (450nm) |
|---|
| 1 | 0 | 2.36 |
| 2 | 5 | 13.5 |
| 3 | 1.5 | 11 |
| 4 | 1.8 | 7 |
| 5 | 1.3 | 4 |
| 6 | 1.8 | 7 |
| 7 | 1.33 | 42 |
| 8 | 1.12 | 14 |
Precision
| Intra assay Precision | Inter assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | TBD | TBD | TBD | TBD | TBD | TBD |
| Mean (ng/ml) | TBD | TBD | TBD | TBD | TBD | TBD |
| Standard Deviation | TBD | TBD | TBD | TBD | TBD | TBD |
| CV(%) | TBD | TBD | TBD | TBD | TBD | TBD |
Linearity
| Serum |
|---|
| 1:2 | Range (%) | TBD |
| Average (%) | 98 | |
| 1:4 | Range (%) | TBD |
| Average (%) | 98 |
Recovery
| Sample Type | Range(%) | Average Recovery(%) |
|---|
| Serum | TBD | TBD |
Description
The Intrinsic Hepcidin IDx™ EDTA ELISA is designed for quantification of hepcidin-25 in human EDTA plasma samples only. This kit does not contain mercaptoethanol, azide- or mercury-based preservatives.
Hepcidin was first discovered as an antimicrobial peptide produced in the liver and then identified as the peptide hormone that regulates extracellular iron in response to changes in dietary and systemic iron load, anemia, hypoxia, and erythropoiesis. Hepcidin is an acute phase protein that is elevated in inflammation with IL-6 acting as the principal regulator of hepcidin during inflammation. Hepcidin inhibits cellular iron efflux by binding to and inducing degradation of the sole known iron channel, ferroportin.
U.S. Pat. No. 9,657,098; NZ Pat. No. 631098; Pat. Pending in the US and abroad.
Questions? Please email us: custserv@intrinsiclifesciences.com
Additional information
| Weight | 10 oz |
|---|---|
| Dimensions | 5 × 7 × 3.5 in |
