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Intrinsic Hepcidin IDx™ ELISA Kit

$838.00

Species: Human Analyte: Hepcidin
Range: 5 – 250 ng/ml Sensitivity: 2.5 ng/ml
SKU: ICE-007 Category: Tags: , ,
Catalog NumberICE-007
Quantity96 Wells
ApplicationELISA, EIA, RUO
MethodCompetitive
Antibody TypeMonoclonal
Target SpeciesHuman
AnalyteHepcidin-25, HAMP, HEPC, LEAP1
Assay time1hr 45m
Detection methodColorimetric
Sample typeSerum
Sample volume required per well20ul
Detection range5-250 ng/ml
Sensitivity2.5ng/ml
Regulatory statusResearch Use Only ELISA
Storage requirements4C

Typical Data

Hepcidin StandardsConcentration (ng/mL)Absorbance (450nm)
02.36
2.52.14
101.61
250.90
500.47
1000.24
2500.10
10000.03

Precision

Intra assay PrecisionInter assay Precision
Sample123123
n1415.514.34.515.514.5
Mean (ng/ml)11.544.54.51.511.522.5
Standard Deviation4.511.511.618.517.514
CV(%)15.511.517.511.517.511.5

Linearity

Serum
1:2Range (%)96-99
1:4Range (%)92-105
1:8
1:16

Recovery

Sample TypeRange(%)Average Recovery(%)
Serum91-9997

Description

The Intrinsic Hepcidin IDx™ ELISA kit is a monoclonal antibody-based assay that binds with high affinity to the N-terminus of hepcidin-25. This antibody also binds low abundance, N-terminus isomers of hepcidin-25 with lower affinities.

Hepcidin is a 25-amino acid peptide hormone that regulates dietary iron absorption from the duodenum, controls recycling of senescent erythrocyte iron by macrophages, and manages iron transport from hepatocytes into plasma. Hepcidin is positively regulated by plasma iron and IL-6 (inflammation, infection) and is suppressed by erythropoiesis via erythroferrone. Abnormally low serum hepcidin is associated with iron deficiency anemia (IDA) and hereditary hemochromatosis, and high serum hepcidin can lead to iron sequestration and to anemia of inflammation (anemia of chronic disease) observed in chronic kidney disease (CKD), rheumatoid arthritis (RA), various cancers, and iron refractory iron deficiency anemia (IRIDA). Hepcidin levels have been shown to be useful in predicting response to oral iron therapy.

U.S. Pat. No. 9,657,098; NZ Pat. No. 631098; Pat. Pending in the US and abroad.

ICE-007 Product Insert v6

Questions? Please email us: custserv@intrinsiclifesciences.com


General Information

Catalog number ICE-007
Quantity 96 Wells
Application ELISA, EIA, RUO
Method Competitive
Antibody type Monoclonal
Target species Human
Analyte Hepcidin-25, HAMP, HEPC, LEAP1
Assay time 1.75 hr
Detection method Colormetric
Sample type Serum
Sample volume required per well 250 ul
Detection range 5 – 250 ng/ML
Sensitivity 2.5 ng/mL
Regulatory Status Research use only ELISA
Storage requirements 2-8 c

Typical data

Hepcidin-25 Standard Concentration (ng/ML) Absorbance (450nm)
1 0 2.36
2 2.5 2.14
3 10 1.61
4 25 0.90
5 50 0.47
6 100 0.25
7 250 0.10
8 1000 0.03

Precision

Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 16 16 16 16 16 16
Mean(ng/ml) 21.8 92.8 219.9 22.2 92.8 222.5
Standard deviation 0.68 2.22 8.89 0.73 24.1 8.56
CV (%) 3.1 2.4 4.0 3.3 2.6 3.8

Linearity

Serum
1:2 Range (%) 96-99
Average (%) 97
1:4 Range (%) 92-1015
Average (%) 98

Recovery

Sample Type Range (%) Average Recovery (%)
Serum 91-109 97

Additional information

Weight 10 oz
Dimensions 7 × 6.0 × 4.0 in