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Intrinsic Rat Hepcidin™ ELISA Kit

$738.00

Species: Rat Analyte: Hepcidin
Range: 1.37 – 330ng/ml Sensitivity: 0.86ng/ml
SKU: RHC-007 Category: Tags: , ,
Catalog NumberRMC-007
Quantity96 Wells
ApplicationELISA, EIA, RUO
MethodCompetitive
Antibody TypePolyclonal
Target SpeciesRat
AnalyteHepcidin
Assay time2h 45m
Detection methodColorimetric
Sample typeSerum
Sample volume required per well6ul
Detection range2.48-330ng/ml
Sensitivity0.86ng/ml
Regulatory statusResearch Use Only ELISA
Storage requirements4C

Typical Data

Hepcidin StandardsConcentration (ng/mL)Absorbance (450nm)

Precision

Intra assay PrecisionInter assay Precision
Sample
n
Mean (ng/ml)
Standard Deviation
CV(%)

Linearity

Serum
1:2
1:4
1:8
1:16

Recovery

Sample TypeRange(%)Average Recovery(%)

Description

The Intrinsic Rat Hepcidin™ ELISA is a test kit designed for quantification of rat Hepcidin in serum.
This kit does not contain mercaptoethanol, azide- or mercury-based preservatives.

This kit is a solid-phase enzyme-linked immunosorbent assay (ELISA), based on the principles of competitive binding. Serum samples, standards, or controls are mixed with buffer containing rat hepcidin biotin conjugate. This mixture is incubated in an anti-rat hepcidin antibody coated ELISA microwell plate. The more rat hepcidin in the sample, the less rat hepcdin biotin conjugate will bind to the antibody coated well due to “competition” for antibody binding sites between native rat hepcidin and synthetic rat hepcidin biotin conjugate. The plate is then washed to remove unbound rat hepcidin biotin conjugate. The rat hepcidin biotin conjugate bound to the antibody is detected with streptavidin conjugated horseradish peroxidase (HRP). The binding of the rat hepcidin biotin conjugate is quantified by the addition of TMB, a chromogenic substrate. The reaction produces a blue color and development is stopped with the addition of a stop solution and the absorbance is read at 450 nm. A standard curve is produced by plotting the concentration of the standard versus the absorbance. The intensity of the color is inversely proportional to the concentration of rat hepcidin in the sample. The total assay run time is less 3 hours.

RHC-007 Product Insert

Researcher’s Guide to Hepcidin Testing

Questions? Please email us: [email protected]


General Information

Catalog number RHC-007
Quantity 96 Wells
Application ELISA, EIA, RUO
Method Competitive
Antibody type Polyclonal
Target species Rat
Analyte Hepcidin
Assay time 2h 45m
Detection method Colorimetric
Sample type Serum
Sample volume required per well 6ul
Detection range 1.37-330 ng/ml
Sensitivity LLOD: 0.86ng/ml       LLOQ: 2.48ng/ml
Regulatory Status Research Use Only ELISA
Storage requirements 2-8 °c

Typical data

Hepcidin Standard Concentration (ng/ML) Absorbance (450nm)
1 1000 0.162
2 333.3 0.275
3 111.1 0.531
4 37.0 1.185
5 12.3 1.918
6 4.1 2.424
7 1.4 2.741
8 0 2.836

Precision

Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 24 24 24 24 24 24
Mean(ng/ml) 84.3 169.7 594.4 84.3 169.7 594.4
Standard deviation 11.6 18.3 43.6 13.6 21.6 50.3
CV (%) 0.14 0.11 0.07 0.16 0.13 0.08

Linearity

Serum
1:2 Range (%) 96-102%
Average (%) 99%
1:4 Range (%) 103-107%
Average (%) 105%

Recovery

Sample Type Range (%) Average Recovery (%)
Serum 93-107 102

Additional information

Weight 13 oz
Dimensions 7.0 × 6.0 × 4.0 in

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