Intrinsic Mouse Erythroferrone™ ELISA Kit

Intrinsic Mouse Erythroferrone™ ELISA Kit

Description

The Intrinsic Mouse ERFETM ELISA is a test kit designed for quantification of mouse Erythroferrone in serum. This kit does not contain mercaptoethanol, azide- or mercury-based preservatives.

Detection of mouse erythroferrone is based on the double antibody sandwich ELISA method. The 96-well microplate assay plate is pre-coated with anti-mouse erythroferrone polyclonal antibody. Mouse serum samples, standards, or controls are mixed with buffer and incubated in the assay plate. Mouse erythroferrone present in the sample binds to the immobilized antibody on the assay plate. The plate is then washed to remove unbound mouse erythroferrone. A 2nd anti-mouse erythroferrone antibody conjugated with biotin is added and followed with HRP-SA conjugate which binds to biotinylated anti-mouse erythroferrone. Optical density (OD) is measured by the addition of TMB, the reaction produces a blue color and development is halted with the addition of the stop solution. The OD is read at 450nm on a microplate reader and a standard curve is prepared by plotting the log concentration of the standard curve versus its corresponding OD. The intensity of the color is proportional to the concentration of ERFE in the sample. Total assay run time is less than 3 hours.

Note:
Based on our studies, serum ERFE concentrations in healthy mice (C57BL/6) were essentially undetectable (below the limit of detection). In a mouse model of ineffective erythropoiesis (beta-thalassemia), serum ERFE concentrations ranged from 1-2 ng/ml.

ERF-200 Product Insert

Questions? Please email us: custserv@intrinsiclifesciences.com

SKU: ERF-200 Category:

$788.00

Quantity
96 Tests (1x96 well)
Application
ELISA
Method
Sandwich
Antibody Type
Polyclonal
Target Species
Mouse
Analyte
Erythroferrone
Sample Type
Serum
Recommended Sample Volume
10 μl
Standard Range
0.063 - 4.0 ng/ml
Regulatory Status
Research Use Only. Not for use in diagnostic procedures.
Stability
3 months from date of shipment. Each kit is assembled prior to shipment and assigned an expiration date.
Storage Requirements
2 - 8 C
Shipping Condition
Ambient
Catalog number
ERF-200

Description

The Intrinsic Mouse ERFETM ELISA is a test kit designed for quantification of mouse Erythroferrone in serum. This kit does not contain mercaptoethanol, azide- or mercury-based preservatives.

Detection of mouse erythroferrone is based on the double antibody sandwich ELISA method. The 96-well microplate assay plate is pre-coated with anti-mouse erythroferrone polyclonal antibody. Mouse serum samples, standards, or controls are mixed with buffer and incubated in the assay plate. Mouse erythroferrone present in the sample binds to the immobilized antibody on the assay plate. The plate is then washed to remove unbound mouse erythroferrone. A 2nd anti-mouse erythroferrone antibody conjugated with biotin is added and followed with HRP-SA conjugate which binds to biotinylated anti-mouse erythroferrone. Optical density (OD) is measured by the addition of TMB, the reaction produces a blue color and development is halted with the addition of the stop solution. The OD is read at 450nm on a microplate reader and a standard curve is prepared by plotting the log concentration of the standard curve versus its corresponding OD. The intensity of the color is proportional to the concentration of ERFE in the sample. Total assay run time is less than 3 hours.

Note:
Based on our studies, serum ERFE concentrations in healthy mice (C57BL/6) were essentially undetectable (below the limit of detection). In a mouse model of ineffective erythropoiesis (beta-thalassemia), serum ERFE concentrations ranged from 1-2 ng/ml.

ERF-200 Product Insert

Questions? Please email us: custserv@intrinsiclifesciences.com

Additional information

Weight 16 oz
Dimensions 7.0 × 7 × 4.0 in
0

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