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Hepcidin Murine-Compete™ ELISA Kit

$738.00

Species: Mouse Analyte: Hepcidin
Range: 0.5 – 333 ng/ml Sensitivity: 0.18 ng/ml
SKU: HMC-001 Category: Tags: , ,
Catalog NumberHMC-001
Quantity96 Wells
ApplicationELISA, EIA, RUO
MethodCompetitive
Antibody TypeMonoclonal
Target SpeciesMouse
AnalyteHepcidin-1, HAMP1, HEPC1
Assay time2h 45m
Detection methodColorimetric
Sample typeSerum
Sample volume required per well12ul
Detection range0.5-333ng/ml
Sensitivity0.18ng/ml
Regulatory statusResearch Use Only ELISA
Storage requirements4C

Typical Data

Hepcidin StandardsConcentration (ng/mL)Absorbance (450nm)

Precision

Intra assay PrecisionInter assay Precision
Sample
n
Mean (ng/ml)
Standard Deviation
CV(%)

Linearity

Serum
1:2
1:4
1:8
1:16

Recovery

Sample TypeRange(%)Average Recovery(%)

Description

The Intrinsic LifeSciences Hepcidin-Murine Compete™ ELISA is designed for quantification of hepcidin-1 (hepc-1) in murine serum and urine. Murine hepcidin-2 (hepc-2) is not detected and does not interfere with hepc-1 measurements using this ELISA. This kit does not contain mercaptoethanol, azide- or mercury-based preservatives.

Hepcidin was discovered to be an antimicrobial peptide produced in the liver and was identified as the peptide hormone that regulates extracellular iron in response to changes in dietary and systemic iron load, anemia, hypoxia, erythropoiesis, and inflammation. Hepcidin inhibits cellular iron efflux by binding to and inducing degradation of the sole known iron channel, ferroportin. Synthetic hepcidin injected into mice binds to ferroportin-rich tissues and rapidly lowers serum iron levels. Data on the normal ranges of circulating levels of the hepcidin-1 protein in common mouse model strains (e.g. Bl/6) are not available currently. Murine hepc-1 peptide levels will scale closely with dietary iron concentration of the feed and will correlate with hepc-1 gene expression measured by qPCR.

HMC-001 Product Insert

Questions? Please email us: custserv@intrinsiclifesciences.com


General Information

Catalog number HMC-001
Quantity 96 Wells
Application ELISA, EIA, RUO
Method Competitive
Antibody type Monoclonal
Target species Mouse
Analyte Hepcidin-1, HAMP1, HEPC1
Assay time 2h 45m
Detection method Colormetric
Sample type Serum
Sample volume required per well 12 ul
Detection range 0.5-333 ng/ml
Sensitivity 0.18 ng/ml
Regulatory Status Research Use Only ELISA
Storage requirements 2-8 c

Typical data

Hepcidin-25 Standard Concentration (ng/ML) Absorbance (450nm)
1 1000 0.065
2 333.3 0.078
3 111.1 0.115
4 37.0 0.212
5 12.3 0.422
6 4.1 0.765
7 1.4 1.106
8 0 1.457

Precision

Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 16 16 16 16 16 16
Mean(ng/ml) 25.2 80.8 202.7 24.5 79.0 204.0
Standard deviation 1.30 2.26 7.15 1.08 1.64 3.04
CV (%) 5.2 2.8 3.5 4.4 2.1 1.5

Linearity

Serum
1:4 Range (%) 90-102%
Average (%) 95%
1:8 Range (%) 90-102%
Average (%) 95%
1:16 Range (%) 85-99%
Average (%) 92%

Recovery

Sample Type Range (%) Average Recovery (%)
Serum 93-107 100

Additional information

Weight 13 oz
Dimensions 7.0 × 6.0 × 4.0 in

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